LOCUS       pLV-[hsa-miR-148b]        9153 bp    DNA     circular     19-APR-2007
SOURCE      
COMMENT     
COMMENT     This file is created by Biosettia Inc.
            http://www.biosettia.com/
FEATURES             Location/Qualifiers
     misc_feature    1807..1929
                     /label=cPPT
     CDS             7074..7934
                     /label=Amp(R)
     promoter        8135..8675
                     /label=pUC ori
     promoter        7..235
                     /label=RSV
     LTR             5679..5913
                     /label=3' LTR
     intron          526..1690
                     /label=Intron
     rep_origin      complement(6488..6943)
                     /label=f1 ori
     LTR             236..416
                     /label=5'LTR
     polyA_signal    5985..6115
                     /label=SV40 pA
     misc_feature    6116..6329
                     /label=SV40 ori
     misc_feature    5000..5596
                     /label=WPRE
     promoter        1933..2328
                     /label=human EF1a promoter
     intron          2361..3618
                     /label=intron
     CDS             3665..4990
                     /label=rPuro (RFP Puro)
     misc_RNA        3110..3408
                     /label=hsa-mir-148b precursor
BASE COUNT     2187 a      2369 c      2462 g      2135 t 
ORIGIN
        1 aagcttaatg tagtcttatg caatactctt gtagtcttgc aacatggtaa cgatgagtta 
       61 gcaacatgcc ttacaaggag agaaaaagca ccgtgcatgc cgattggtgg aagtaaggtg 
      121 gtacgatcgt gccttattag gaaggcaaca gacgggtctg acatggattg gacgaaccac 
      181 tgaattgccg cattgcagag atattgtatt taagtgccta gctcgataca taaacgggtc 
      241 tctctggtta gaccagatct gagcctggga gctctctggc taactaggga acccactgct 
      301 taagcctcaa taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga 
      361 ctctggtaac tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcagtgg 
      421 cgcccgaaca gggacttgaa agcgaaaggg aaaccagagg agctctctcg acgcaggact 
      481 cggcttgctg aagcgcgcac ggcaagaggc gaggggcggc gactggtgag tacgccaaaa 
      541 attttgacta gcggaggcta gaaggagaga gatgggtgcg agagcgtcag tattaagcgg 
      601 gggagaatta gatcgcgatg ggaaaaaatt cggttaaggc cagggggaaa gaaaaaatat 
      661 aaattaaaac atatagtatg ggcaagcagg gagctagaac gattcgcagt taatcctggc 
      721 ctgttagaaa catcagaagg ctgtagacaa atactgggac agctacaacc atcccttcag 
      781 acaggatcag aagaacttag atcattatat aatacagtag caaccctcta ttgtgtgcat 
      841 caaaggatag agataaaaga caccaaggaa gctttagaca agatagagga agagcaaaac 
      901 aaaagtaaga ccaccgcaca gcaagcggcc gctgatcttc agacctggag gaggagatat 
      961 gagggacaat tggagaagtg aattatataa atataaagta gtaaaaattg aaccattagg 
     1021 agtagcaccc accaaggcaa agagaagagt ggtgcagaga gaaaaaagag cagtgggaat 
     1081 aggagctttg ttccttgggt tcttgggagc agcaggaagc actatgggcg cagcgtcaat 
     1141 gacgctgacg gtacaggcca gacaattatt gtctggtata gtgcagcagc agaacaattt 
     1201 gctgagggct attgaggcgc aacagcatct gttgcaactc acagtctggg gcatcaagca 
     1261 gctccaggca agaatcctgg ctgtggaaag atacctaaag gatcaacagc tcctggggat 
     1321 ttggggttgc tctggaaaac tcatttgcac cactgctgtg ccttggaatg ctagttggag 
     1381 taataaatct ctggaacaga tttggaatca cacgacctgg atggagtggg acagagaaat 
     1441 taacaattac acaagcttaa tacactcctt aattgaagaa tcgcaaaacc agcaagaaaa 
     1501 gaatgaacaa gaattattgg aattagataa atgggcaagt ttgtggaatt ggtttaacat 
     1561 aacaaattgg ctgtggtata taaaattatt cataatgata gtaggaggct tggtaggttt 
     1621 aagaatagtt tttgctgtac tttctatagt gaatagagtt aggcagggat attcaccatt 
     1681 atcgtttcag acccacctcc caaccccgag gggacccgac aggcccgaag gaatagaaga 
     1741 agaaggtgga gagagagaca gagacagatc cattcgatta gtgaacggat ctcgacggta 
     1801 tcggttaact tttaaaagaa aaggggggat tggggggtac agtgcagggg aaagaatagt 
     1861 agacataata gcaacagaca tacaaactaa agaattacaa aaacaaatta caaaattcaa 
     1921 aattttatcg atgcctcccc gtcaccaccc cccccaaccc gccccgaccg gagctgagag 
     1981 taattcatac aaaaggactc gcccctgcct tggggaatcc cagggaccgt cgttaaactc 
     2041 ccactaacgt agaacccaga gatcgctgcg ttcccgcccc ctcacccgcc cgctctcgtc 
     2101 atcactgagg tggagaagag catgcgtgag gctccggtgc ccgtcagtgg gcagagcgca 
     2161 catcgcccac agtccccgag aagttggggg gaggggtcgg caattgaacc ggtgcctaga 
     2221 gaaggtggcg cggggtaaac tgggaaagtg atgtcgtgta ctggctccgc ctttttcccg 
     2281 agggtggggg agaaccgtat ataagtgcag tagtcgccgt gaacgttctt tttcgcaacg 
     2341 ggtttgccgc cagaacacag gtaagtgccg tgtgtggttc ccgcgggcct ggcctcttta 
     2401 cgggttatgg cccttgcgtg ccttgaatta cttccacgcc cctggctgca gtacgtgatt 
     2461 cttgatcccg agcttcgggt tggaagtggg tgggagagtt cgaggccttg cgcttaagga 
     2521 gccccttcgc ctcgtgcttg agttgaggcc tggcctgggc gctggggccg ccgcgtgcga 
     2581 atctggtggc accttcgcgc ctgtctcgct gctttcgata agtctctagc catttaaaat 
     2641 ttttgatgat atcctgcgac gctttttttc tggcaagata gtcttgtaaa tgcgggccaa 
     2701 gatctgcaca ctggtatttc ggtttttggg gccgcgggcg gcgacggggc ccgtgcgtcc 
     2761 cagcgcacat gttcggcgag gcggggcctg cgagcgcggc caccgagaat cggacggggg 
     2821 tagtctcaag ctggccggcc tgctctggtg cctggcctcg cgccgccgtg tatcgccccg 
     2881 ccctgggcgg caaggctggc ccggtcggca ccagttgcgt gagcggaaag atggccgctt 
     2941 cccggccctg ctgcagggag ctcaaaatgg aggacgcggc gctcgggaga gcgggcgggt 
     3001 gagtcaccca cacaaaggaa aagggccttt ccgtcctcag ccgtcgcttc atgtgactcc 
     3061 acggagtacc gggcgccgtc caggcacctc gattagttct cgaggatccg gacagcattc 
     3121 ttgttttctt gataataaaa acataaatac atgaaaatct gtctaagtca cccaatctcc 
     3181 cacaaaacaa tctgcctata catcatttcc aagcacgatt agcatttgag gtgaagttct 
     3241 gttatacact caggctgtgg ctctctgaaa gtcagtgcat cacagaactt tgtctcgaaa 
     3301 gctttctagc agctacccat tttgggagtg ggagggaaga atagaccttt taaattcttt 
     3361 cagtgtggcc ctaagctgat aaggtctttc tcaacagtca gcatttaagc tagctcgagc 
     3421 ttttggagta cgtcgtcttt aggttggggg gaggggtttt atgcgatgga gtttccccac 
     3481 actgagtggg tggagactga agttaggcca gcttggcact tgatgtaatt ctccttggaa 
     3541 tttgcccttt ttgagtttgg atcttggttc attctcaagc ctcagacagt ggttcaaagt 
     3601 ttttttcttc catttcaggt gtcgtgaaaa ctacccctct agagtcgagc taccggtcgc 
     3661 caccatggtg agcaagggcg aggaggataa catggccatc atcaaggagt tcatgcgctt 
     3721 caaggtgcac atggagggct ccgtgaacgg ccacgagttc gagatcgagg gcgagggcga 
     3781 gggccgcccc tacgagggca cccagaccgc caagctgaag gtgaccaagg gtggccccct 
     3841 gcccttcgcc tgggacatcc tgtcccctca gttcatgtac ggctccaagg cctacgtgaa 
     3901 gcaccccgcc gacatccccg actacttgaa gctgtccttc cccgagggct tcaagtggga 
     3961 gcgcgtgatg aacttcgagg acggcggcgt ggtgaccgtg acccaggact cctccctgca 
     4021 ggacggcgag ttcatctaca aggtgaagct gcgcggcacc aacttcccct ccgacggccc 
     4081 cgtaatgcag aagaagacca tgggctggga ggcctcctcc gagcggatgt accccgagga 
     4141 cggcgccctg aagggcgaga tcaagcagag gctgaagctg aaggacggcg gccactacga 
     4201 cgctgaggtc aagaccacct acaaggccaa gaagcccgtg cagctgcccg gcgcctacaa 
     4261 cgtcaacatc aagttggaca tcacctccca caacgaggac tacaccatcg tggaacagta 
     4321 cgaacgcgcc gagggccgcc actccaccgg cggcatggac gagctgtaca aggacccacc 
     4381 ggtcgccacc atgaccgagt acaagcccac ggtgcgcctc gccacccgcg acgacgtccc 
     4441 cagggccgta cgcaccctcg ccgccgcgtt cgccgactac cccgccacgc gccacaccgt 
     4501 cgatccggac cgccacatcg agcgggtcac cgagctgcaa gaactcttcc tcacgcgcgt 
     4561 cgggctcgac atcggcaagg tgtgggtcgc ggacgacggc gccgcggtgg cggtctggac 
     4621 cacgccggag agcgtcgaag cgggggcggt gttcgccgag atcggcccgc gcatggccga 
     4681 gttgagcggt tcccggctgg ccgcgcagca acagatggaa ggcctcctgg cgccgcaccg 
     4741 gcccaaggag cccgcgtggt tcctggccac cgtcggcgtc tcgcccgacc accagggcaa 
     4801 gggtctgggc agcgccgtcg tgctccccgg agtggaggcg gccgagcgcg ccggggtgcc 
     4861 cgccttcctg gagacctccg cgccccgcaa cctccccttc tacgagcggc tcggcttcac 
     4921 cgtcaccgcc gacgtcgagt gcccgaagga ccgcgcgacc tggtgcatga cccgcaagcc 
     4981 cggtgcctga gcggccgcgt cgacaatcaa cctctggatt acaaaatttg tgaaagattg 
     5041 actggtattc ttaactatgt tgctcctttt acgctatgtg gatacgctgc tttaatgcct 
     5101 ttgtatcatg ctattgcttc ccgtatggct ttcattttct cctccttgta taaatcctgg 
     5161 ttgctgtctc tttatgagga gttgtggccc gttgtcaggc aacgtggcgt ggtgtgcact 
     5221 gtgtttgctg acgcaacccc cactggttgg ggcattgcca ccacctgtca gctcctttcc 
     5281 gggactttcg ctttccccct ccctattgcc acggcggaac tcatcgccgc ctgccttgcc 
     5341 cgctgctgga caggggctcg gctgttgggc actgacaatt ccgtggtgtt gtcggggaag 
     5401 ctgacgtcct ttccatggct gctcgcctgt gttgccacct ggattctgcg cgggacgtcc 
     5461 ttctgctacg tcccttcggc cctcaatcca gcggaccttc cttcccgcgg cctgctgccg 
     5521 gctctgcggc ctcttccgcg tcttcgcctt cgccctcaga cgagtcggat ctccctttgg 
     5581 gccgcctccc cgcctggaat tcgagctcgg tacctttaag accaatgact tacaaggcag 
     5641 ctgtagatct tagccacttt ttaaaagaaa aggggggact ggaagggcta attcactccc 
     5701 aacgaagaca agatctgctt tttgcttgta ctgggtctct ctggttagac cagatctgag 
     5761 cctgggagct ctctggctaa ctagggaacc cactgcttaa gcctcaataa agcttgcctt 
     5821 gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc tggtaactag agatccctca 
     5881 gaccctttta gtcagtgtgg aaaatctcta gcagtagtag ttcatgtcat cttattattc 
     5941 agtatttata acttgcaaag aaatgaatat cagagagtga gaggaacttg tttattgcag 
     6001 cttataatgg ttacaaataa agcaatagca tcacaaattt cacaaataaa gcattttttt 
     6061 cactgcattc tagttgtggt ttgtccaaac tcatcaatgt atcttatcat gtctggctct 
     6121 agctatcccg cccctaactc cgcccatccc gcccctaact ccgcccagtt ccgcccattc 
     6181 tccgccccat ggctgactaa ttttttttat ttatgcagag gccgaggccg cctcggcctc 
     6241 tgagctattc cagaagtagt gaggaggctt ttttggaggc ctagggacgt acccaattcg 
     6301 ccctatagtg agtcgtatta cgcgcgctca ctggccgtcg ttttacaacg tcgtgactgg 
     6361 gaaaaccctg gcgttaccca acttaatcgc cttgcagcac atcccccttt cgccagctgg 
     6421 cgtaatagcg aagaggcccg caccgatcgc ccttcccaac agttgcgcag cctgaatggc 
     6481 gaatgggacg cgccctgtag cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc 
     6541 gtgaccgcta cacttgccag cgccctagcg cccgctcctt tcgctttctt cccttccttt 
     6601 ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc gggggctccc tttagggttc 
     6661 cgatttagtg ctttacggca cctcgacccc aaaaaacttg attagggtga tggttcacgt 
     6721 agtgggccat cgccctgata gacggttttt cgccctttga cgttggagtc cacgttcttt 
     6781 aatagtggac tcttgttcca aactggaaca acactcaacc ctatctcggt ctattctttt 
     6841 gatttataag ggattttgcc gatttcggcc tattggttaa aaaatgagct gatttaacaa 
     6901 aaatttaacg cgaattttaa caaaatatta acgcttacaa tttaggtggc acttttcggg 
     6961 gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc 
     7021 tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta 
     7081 ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg 
     7141 ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg 
     7201 gttacatcga actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac 
     7261 gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtattg 
     7321 acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt 
     7381 actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg 
     7441 ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac 
     7501 cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt 
     7561 gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgtag 
     7621 caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc 
     7681 aacaattaat agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc 
     7741 ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta 
     7801 tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg 
     7861 ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga 
     7921 ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac 
     7981 ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa 
     8041 tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat 
     8101 cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc 
     8161 taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg 
     8221 gcttcagcag agcgcagata ccaaatactg ttcttctagt gtagccgtag ttaggccacc 
     8281 acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg 
     8341 ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg 
     8401 ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa 
     8461 cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg 
     8521 aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga 
     8581 gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct 
     8641 gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca 
     8701 gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc 
     8761 ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg 
     8821 ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc 
     8881 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca 
     8941 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc 
     9001 attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga 
     9061 gcggataaca atttcacaca ggaaacagct atgaccatga ttacgccaag cgcgcaatta 
     9121 accctcacta aagggaacaa aagctggagc tgc 
//
